HiHo-AID2: boosting homozygous knock-in efficiency enables robust generation of human auxin-inducible degron cells
Li S, Wang Y, van der Stoel M, Zhou X, Madhusudan S, Kanerva K, Nguyen VD, Eskici N, Olkkonen VM, Zhou Y, Raivio T, Ikonen E
Genome Biol. 2024; 25, 58. 10.1186/s13059-024-03187-w Article
Li S, Wang Y, van der Stoel M, Zhou X, Madhusudan S, Kanerva K, Nguyen VD, Eskici N, Olkkonen VM, Zhou Y, Raivio T, Ikonen E
Genome Biol. 2024; 25, 58. 10.1186/s13059-024-03187-w Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 216243 | pGL3-EF1a-i53 | Express i53 in mammalian cells as a HDR enhancer |
| 216244 | pSH-CAG-AtAFB2.F74A-mCherry-IRES-puro | Express AtAFB2.F74A mutant with mCherry tag for AID2 |
| 216245 | pSH-CAG-AtAFB2.F74A-IRES-puro | Express AtAFB2.F74A mutant without tag for AID2 |
| 216246 | pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro | Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration |
| 216247 | pSH-EF1a-OsTIR1.F74A-mCherry-IRES-puro | Express OsTIR1.F74A mutant with mCherry tag for AID2 |
| 216248 | pGL3-RANGAP.C-linker.miniIAA7.3xFlag.P2A.BSD | HDR template to tag endogenous human RANGAP1 C-terminus with miniIAA7.3xFlag.P2A.BSD |
| 216249 | pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro | Express Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminus |
| 216250 | pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag | HDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlag |
| 216251 | pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro | Express Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminus |