A color-tailored fluorogenic sensor for hydrogen peroxide.
Potekhina ES, Bass DI, Ezerina D, Fleckenstein DD, Chebotarev AS, Sysoeva VA, Maltsev DI, Pak VV, Moshchenko AA, Sokolov AI, Myasnyanko IN, Baranov MS, Fedotov AB, Zheltikov AM, Lanin AA, Messens J, Belousov VV
Nat Chem Biol. 2025 Oct 16. doi: 10.1038/s41589-025-02036-6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 216676 | pD441-H6-SUMO-HyPerFLEX | Plasmid with cleavable His-tag for expression of the HyPer-FLEX sensor for hydrogen peroxide in E. coli |
| 217562 | AAV-CAG-HyPerFLEX-NES-WPRE | Mammalian expression of HyPerFLEX targeted to the cytoplasm |
| 217563 | AAV-CAG-HyPerFLEX-NLSx3-WPRE | Mammalian expression of HyPerFLEX targeted to the nucleus |
| 217564 | AAV-CaMKII-HyPerFLEX-UnaG-WPRE | Expression of HyPerFLEX fused with UnaG in mammalian neurons |
| 217565 | AAV-CMV-HyPerFLEX-UnaG-WPRE | Mammalian expression of HyPerFLEX fused with UnaG |
| 217566 | pAAV-CaMKII-mTagBFP2-P2A-DAAO-NES | Expression of DAAO targeted to the cytoplasm and mTagBFP2, separated by P2A self-cleaving peptide, in mammalian neurons |
| 217567 | pCS2+-tdmito-HyPerFLEX | Mammalian expression HyPerFLEX targeted to the mitochondria |
| 217568 | pSC2+-HyPerFLEX-Lifeact | Mammalian expression HyPerFLEX targeted to actin filaments |
| 217569 | pQE30-HyPerFLEX | Bacterial expression of HyPerFLEX |
| 238940 | pAAV-CMV-HyPerFLEX-Sec61b | Mammalian expression of HyPerFLEX targeted to the cytosolic side of the endoplasmic reticulum membranes |
| 238941 | pC1-HyPerFLEX-ERlum | Mammalian expression of HyPerFLEX targeted to the luminal side of the endoplasmic reticulum membranes |