Construction and Characterization of MoClo-Compatible Vectors for Modular Protein Expression in E. coli.
Nielsen JR, Lewis MJ, Huang WE
ACS Synth Biol. 2025 Jan 12. doi: 10.1021/acssynbio.4c00564.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 217582 | DVK_p15A_AE | Destination vector with kanamycin resistance and p15A origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning. |
| 217583 | DVK_p15A_AF | Destination vector with kanamycin resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning. |
| 217584 | DVK_p15A_EF | Destination vector with kanamycin resistance and p15A origin of replication. Carries E-F CIDAR MoClo overhangs for level 1 construct cloning. |
| 217585 | DVK_p15A_FG | Destination vector with kanamycin resistance and p15A origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning. |
| 217586 | DVK_p15A_GH | Destination vector with kanamycin resistance and p15A origin of replication. Carries G-H CIDAR MoClo overhangs for level 1 construct cloning. |
| 217587 | DVC_p15A_AE | Destination vector with chloramphenicol resistance and p15A origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning. |
| 217588 | DVC_p15A_AF | Destination vector with chloramphenicol resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning. |
| 217589 | DVC_p15A_EF | Destination vector with chloramphenicol resistance and p15A origin of replication. Carries E-F CIDAR MoClo overhangs for level 1 construct cloning. |
| 217590 | DVC_p15A_FG | Destination vector with chloramphenicol resistance and p15A origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning. |
| 217591 | DVC_p15A_GH | Destination vector with chloramphenicol resistance and p15A origin of replication. Carries G-H CIDAR MoClo overhangs for level 1 construct cloning. |
| 217592 | DVK_pBR322_AE | Destination vector with kanamycin resistance and pBR322 origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning. |
| 217593 | DVK_pBR322_AF | Destination vector with kanamycin resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning. |
| 217594 | DVK_pBR322_EF | Destination vector with kanamycin resistance and pBR322 origin of replication. Carries E-F CIDAR MoClo overhangs for level 1 construct cloning. |
| 217595 | DVK_pBR322_FG | Destination vector with kanamycin resistance and pBR322 origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning. |
| 217596 | DVK_pBR322_GH | Destination vector with kanamycin resistance and pBR322 origin of replication. Carries G-H CIDAR MoClo overhangs for level 1 construct cloning. |
| 217597 | DVC_pBR322_AE | Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning. |
| 217598 | DVC_pBR322_AF | Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning. |
| 217599 | DVC_pBR322_EF | Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries E-F CIDAR MoClo overhangs for level 1 construct cloning. |
| 217600 | DVC_pBR322_FG | Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning. |
| 217601 | DVC_pBR322_GH | Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries G-H CIDAR MoClo overhangs for level 1 construct cloning. |
| 217602 | DVA_p15A_AH | Destination vector with ampicillin resistance and p15A origin of replication. Carries A-H CIDAR MoClo overhangs for level 0 or 2 construct cloning. |
| 217603 | DVA_p15A_AG | Destination vector with ampicillin resistance and p15A origin of replication. Carries A-G CIDAR MoClo overhangs for level 0 or 2 construct cloning. |
| 217604 | DVA_p15A_AF | Destination vector with ampicillin resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 0 or 2 construct cloning. |
| 217605 | DVA_p15A_EH | Destination vector with ampicillin resistance and p15A origin of replication. Carries E-H CIDAR MoClo overhangs for level 0 or 2 construct cloning. |
| 217606 | DVA_pBR322_AH | Destination vector with ampicillin resistance and pBR322 origin of replication. Carries A-H CIDAR MoClo overhangs for level 0 or 2 construct cloning. |
| 217607 | DVA_pBR322_AG | Destination vector with ampicillin resistance and pBR322 origin of replication. Carries A-G CIDAR MoClo overhangs for level 0 or 2 construct cloning. |
| 217608 | DVA_pBR322_AF | Destination vector with ampicillin resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 0 or 2 construct cloning. |
| 217609 | DVA_pBR322_EH | Destination vector with ampicillin resistance and pBR322 origin of replication. Carries E-H CIDAR MoClo overhangs for level 0 or 2 construct cloning. |