CRISPR screening by AAV episome-sequencing (CrAAVe-seq): a scalable cell-type-specific in vivo platform uncovers neuronal essential genes.

Ramani B, Rose IVL, Teyssier N, Pan A, Danner-Bocks S, Sanghal T, Yadanar L, Tian R, Ma K, Palop JJ, Kampmann M
Nat Neurosci. 2025 Aug 22. doi: 10.1038/s41593-025-02043-9. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
217635	pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2	AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.