A structure-based mechanism for initiation of AP-3 coated vesicle formation.
Begley M, Aragon M, Baker RW
Proc Natl Acad Sci U S A. 2024 Dec 24;121(52):e2411974121. doi: 10.1073/pnas.2411974121. Epub 2024 Dec 20.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 217834 | MZB035 | Bacterial expression of human AP3D1 (1-617) and AP3S1 AP-3 core hemicomplex |
| 217835 | MZB053 | Bacterial expression of human AP3B1 (1-677) and AP3M1 AP-3 core hemicomplex |
| 217836 | MZB127 | Bacterial expression of human Arf1 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag |
| 217838 | MZB139 | Empty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression |
| 217839 | MZB131 | Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged AP3D1 adaptin appendage (920-1153) for pulldown assays |
| 217840 | MZB150 | Bacterial expression of 10xHis-SUMO (Smt3) fusion to Sec7 domain (52-252) of Cytohesin-2 (CYTH2/ARNO) for rapid nucleotide exchange and activation of Arf GTPases |
| 217841 | MZB160 | Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged Arf (L8K+Q71L) for pulldown assays |
| 217842 | MZB164 | 10xHis-SUMO (Smt3) fused, HRV-3C cleavable, Halotag for rapid cloning/DNA assembly of pulldown targets via SwaI restriction site for E. coli based protein expression |
| 217843 | MZB207 | Bacterial expression of 10xHis-SUMO (Smt3) fusion to AP3D1 amphipathic helix (2-15) fused to mGreenLantern |
| 217844 | MZB208 | Bacterial expression of 10xHis-SUMO (Smt3) fusion to AP3M1 amphipathic helix (135-151) fused to mGreenLantern |
| 221766 | MZB216 | "Empty" C-terminal CPD-10xHis vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression |
| 221767 | MZB215 | Bacterial expression of human Arf6 (Q67L) with C-terminal 'self-cleaving' CPD-10xHis tag |
| 221769 | MZB218 | Bacterial expression of human Arf4 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag |
| 221770 | MZB219 | Bacterial expression of human Arf5 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag |
| 221771 | MZB220 | Bacterial expression of human Arl1 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag |
| 221772 | MZB222 | Bacterial expression of 10xHis-SUMO (Smt3) fusion to Sec7 domain (687-885) of Big1 (ArfGEF1) for rapid nucleotide exchange and activation of Arf GTPases |
| 226998 | MZB224 | Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged Arf (T31N) for pulldown assays |