Skip to main content
Addgene
Open Menu

Insulated piggyBac and FRT vectors for engineering transgenic homozygous and heterozygous eHAP cells.

Minard AY, Winistorfer S, Piper RC
Biol Open. 2025 Oct 15;14(10):bio061793. doi: 10.1242/bio.061793. Epub 2025 Oct 1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
217970pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLSProvides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.
217971pPL7552_ColE1_PiggyBac_2xcHS4::mCherry-IRES-PuroR-P2A-mTagBFP2-NLS::2xcHS4Provides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.

Antibodies from Article