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Fucosylated glycoproteins and fucosylated glycolipids play opposing roles in cholera intoxication.

Ghorashi AC, Boucher A, Archer-Hartmann SA, Murray NB, Konada RSR, Zhang X, Xing C, Azadi P, Yrlid U, Kohler JJ
bioRxiv [Preprint]. 2023 Aug 3:2023.08.02.551727. doi: 10.1101/2023.08.02.551727. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
208380lentiMutB3GNT5-blastlentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
208381lentiMutB3GALT5-blastlentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
220867lentiGuideB3GNT5-neoencodes CRISPR sgRNA targeting human B3GNT5
220868lentiGuideSLC35C1-neoencodes CRISPR sgRNA targeting human SLC3C1
220869lentiMutB3GALT5catdead-blastlentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

Antibodies from Article