AAV-compatible optogenetic tools for activating endogenous calcium channels in vivo.
Kook YH, Lee H, Lee J, Jeong Y, Rho J, Heo WD, Lee S
Mol Brain. 2023 Oct 17;16(1):73. doi: 10.1186/s13041-023-01061-7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 248293 | pCMV-FLAG-CRY2-STIM1(238-685) | An expression construct encoding a CRY2-fused FLAG-tagged STIM1 fragment (a.a.238–685). |
| 248294 | pCMV-EGFP-CRY2-STIM1(318-450) | An expression construct encoding a CRY2-fused truncated STIM1 fragment (a.a.318–450). |
| 248295 | pCMV-EGFP-IRES2-CRY2-STIM1(238-448) | An expression construct encoding a CRY2-fused STIM1 fragment (a.a.238-448) linked to EGFP through an IRES2 element. |
| 248296 | pCMV-CIBN-STIM1(238-685) | An expression construct encoding a CIBN-fused STIM1 fragment (a.a.238-685). |
| 248297 | pCMV-EGFP-STIM1(238-685) | An expression construct encoding an EGFP-fused STIM1 fragment (a.a.238-685). |
| 248298 | pCMV-vhhGFP-CRY2 | An expression construct encoding a GFP nanobody (vhhGFP) fused to CRY2. |
| 248299 | pAAV-CaMKIIα-FLAG-STIM1(238-685) | An AAV construct designed to express a CRY2-fused FLAG-tagged STIM1 fragment (a.a.238-685) under the control of the CaMKIIα promoter. |
| 248301 | pAAV-GfaABC1D-FLAG-STIM1(238-685) | An AAV construct designed to express a CRY2-fused FLAG-tagged STIM1 fragment (a.a.238-685) under the control of the GfaABC1D promoter. |
| 248302 | pAAV-GfaABC1D-EGFP-CRY2-STIM1(318-450) | An AAV construct designed to express a CRY2-fused truncated STIM1 fragment (a.a.318–450) under the control of the GfaABC1D promoter. |