High-Density Guide RNA Tiling and Machine Learning for Designing CRISPR Interference in Synechococcus sp. PCC 7002.
Dallo T, Krishnakumar R, Kolker SD, Ruffing AM
ACS Synth Biol. 2023 Apr 21;12(4):1175-1186. doi: 10.1021/acssynbio.2c00653. Epub 2023 Mar 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 191092 | pNS1D-sgRNA9-SpR | aTc inducible gRNA expression plasmid with BbsI sites for Golden Gate insertion of crRNA oligos and homology arms for genome integration at NS1D in Synechococcus sp. PCC 7002 |