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Intracellular tension sensor reveals mechanical anisotropy of the actin cytoskeleton.

Amiri S, Muresan C, Shang X, Huet-Calderwood C, Schwartz MA, Calderwood DA, Murrell M
Nat Commun. 2023 Dec 4;14(1):8011. doi: 10.1038/s41467-023-43612-5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
234868pLenti-CMV-puro_FlnaABD-TS-16flagExpresses control FlnA-based tension sensor that lacks dimerization domain and therefore does not report tension.
234869pLenti-CMV-puro_FlnaABD-15-TS-16-24flagExpresses the FlnA actin binding domain and Ig15 , a FRET construct containing GFP-F40-RFP, the FlnA Ig 16 and Ig 24 domains.

Antibodies from Article