A Cas3-base editing tool for targetable in vivo mutagenesis.
Zimmermann A, Prieto-Vivas JE, Cautereels C, Gorkovskiy A, Steensels J, Van de Peer Y, Verstrepen KJ
Nat Commun. 2023 Jun 9;14(1):3389. doi: 10.1038/s41467-023-39087-z.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 229534 | pCas3-CDA1 | pMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning |
| 229535 | pdnCas3-CDA1 | pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning |
| 229536 | pCas3-APOBEC1 | pMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning |
| 229537 | pdnCas3-APOBEC1 | pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning |
| 229538 | pCas3 | pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning. |