Multiplex, single-cell CRISPRa screening for cell type specific regulatory elements.
Chardon FM, McDiarmid TA, Page NF, Daza RM, Martin BK, Domcke S, Regalado SG, Lalanne JB, Calderon D, Li X, Starita LM, Sanders SJ, Ahituv N, Shendure J
Nat Commun. 2024 Sep 18;15(1):8209. doi: 10.1038/s41467-024-52490-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 218234 | piggyFlex | A piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers. |