Combinatorial protein engineering identifies potent CRISPR activators with reduced toxicity.
Giddins M, Kratz AF, De Los Santos MB, Forget A, Tiwari R, Jang G, Blazejewski T, Qin C, Huang Y, Lao YH, Falconer T, Leong KW, Krogan N, Staller M, Wang H, Wei L, Chavez A
Nat Commun. 2025 Nov 20;16(1):11114. doi: 10.1038/s41467-025-65986-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 218555 | pb-CAGGS-dCas9-blasto | Encodes CAG promoter-driven dCas9 with a C-terminal bipartite SV40 NLS, along with blasticidin resistance |
| 218556 | pb-CAGGS-dCas9-VP64-blasto | Encodes CAG promoter-driven dCas9-VP64 with a bipartite SV40 NLS between dCas9 and VP64, along with blasticidin resistance |
| 218557 | pLex307-UCOE-SFFV-puroR-MCP-HSF1-VIRF2 | Encodes SFFV promoter-driven MCP-HSF1-VIRF2 with an N-terminal bipartite SV40 NLS, along with puromycin resistance |
| 218558 | pLex307-UCOE-SFFV-puroR-MCP-MLLx3-HSF1 | Encodes SFFV promoter-driven MCP-MLLx3-HSF1 with an N-terminal bipartite SV40 NLS, along with puromycin resistance |
| 218559 | pLex307-UCOE-SFFV-puroR-MCP-P65-HSF1 | Encodes SFFV promoter-driven MCP-P65-HSF1 with an N-terminal bipartite SV40 NLS, along with puromycin resistance |