Systematic comparison of dCas9-based DNA methylation epimodifiers over time indicates efficient on-target and widespread off-target effects
Kakhki MP, Rangani F, Ewing E, Cucuzza CS, Zheleznyakova G, Kalomoiri M, Badam TVS, Covacu R, Andreou I, Needhamsen M, Kular L, Jagodic M
bioRxiv 2025.03.15.641804; doi: 10.1101/2025.03.15.641804
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 218776 | M3-dCAS9-DNMT3A-DNMT3A (dCas9-3A-3A) | Expresses dCAS9-DNMT3A-DNMT3A fusion protein and double marker GFP-T2A-Puro under independent promoters |
| 218777 | M3-dCAS9-DNMT3A-DNMT3L (dCas9-3A-3L) | Expresses dCAS9-DNMT3A-DNMT3L fusion protein and double marker GFP-T2A-Puro under independent promoters |
| 218781 | M3-dCAS9-DNMT3A-KRAB (dCas9-3A-KRAB) | Expresses dCAS9-DNMT3A-KRAB fusion protein and double marker GFP-T2A-Puro under independent promoters |
| 218782 | M3-dCAS9-M.SssI (dCas9-M.SssI) | Expresses dCAS9-M.SssI fusion protein and double marker GFP-T2A-Puro under independent promoters |
| 218783 | M3-dCAS9-SunTag (dCas9-SunTag) | Expresses dCAS9-SunTag fusion protein and double marker GFP-T2A-Puro under independent promoters |
| 218787 | M3-dCAS9-dDNMT3A (dCas9-d3A) | Expresses dCAS9-dead DNMT3A fusion protein and double marker GFP-T2A-Puro under independent promoters |
| 218788 | M3-dCAS9-mutDNMT3A(R882H)(dCas9-mut3A) | Expresses dCAS9- R882H Mutated DNMT3A fusion protein and double marker GFP-T2A-Puro under independent promoters |
| 220237 | M3-dCAS9-DNMT3A | Expresses dCAS9-DNMT3A fusion protein and double marker GFP-T2A-Puro under independent promoters |