Rigid enlargement of sybodies with antibody fragments for cryo-EM analyses of small membrane proteins.
Ackle F, Thavarasah S, Earp JC, Seeger MA
Sci Rep. 2025 Mar 19;15(1):9460. doi: 10.1038/s41598-025-92950-5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 219967 | pSBLego | The pSBLego vector enables sybodies for the Legobody method with a PelB signal for periplasmic expression and a "SLEHHHHHH" motif for purification and Legobody Fab binding. |