Base editing both DNA strands in distinct editing windows with small CRISPR-associated effector Cas12f1.
Swartjes T, Bouzetos E, Adiego-Perez B, Pool VD, Staals RHJ, van der Oost J, Wu WY
iScience. 2025 Nov 12;28(12):114033. doi: 10.1016/j.isci.2025.114033. eCollection 2025 Dec 19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220993 | pdCas12f1-LVA | Prokaryotic and constitutive expression of AsCas12f-LVA |
| 220994 | pdCas12f1_CBE-LVA | Prokaryotic and constitutive expression of AsCas12f1/APOBEC1-LVA fusion |
| 220995 | pdCas12f1Cas_ABE-LVA | Prokaryotic and constitutive expression of AsCas12f1/TadA8e-LVA fusion |
| 220996 | pTarget-entry | Prokaryotic expression of mRFP and GFP. Target sequences are inserted in place of the RFP gene through AatII/KpnI restriction ligation |