Rab8 and TNPO1 are ciliary transport adaptors for GTPase Arl13b by interacting with its RVEP motif containing ciliary targeting sequence.
Mahajan D, Madugula V, Lu L
J Biol Chem. 2023 May;299(5):104604. doi: 10.1016/j.jbc.2023.104604. Epub 2023 Mar 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 221161 | Arl13b-RVEP-4A-GFP | Expresses mammalian Arl13b with RVEP4A mutation and GFP-tagged |
| 221175 | PAL-GFP | Expresses the palmitolylation region of Arl13b and GFP-tagged |
| 221176 | PAL-AA248-428-GFP | Expresses the palmitolylation region and amino acids 248-428 of mamalian Arl13b and GFP-tagged |
| 221591 | PAL-AA246–324-GFP | Expresses the palmitolylation region of mamalian Arl13b (amino acids 246-325) and GFP-tagged |
| 221592 | PAL-13bCTS-GFP | Expresses the palmitoylation sequence, the ciliary targeting sequence of mammalian Arl13b (amino acids 347-363) and GFP-tagged |
| 221593 | PAL-AA347-363-RVEP-4A-GFP | Expresses the palmitoylation sequence, the ciliary targeting sequence with RVEP4A mutation of mammalian Arl13b (amino acids 347-363) and GFP-tagged |
| 221596 | CD8a-AA347-363-GFP | Expresses CD8a with ciliary targeting sequence (CTS) from amino acids 347-363 of Arl13b fused to the cytosolic tail and GFP-tagged |
| 221597 | CD8a-AA347-363- RVEP-4A-GFP | Expresses CD8a with of Arl13b ciliary targeting sequence (CTS) from amino acids 347-363 with RVEP4A mutation fused to the cytosolic tail and GFP-tagged |
| 221598 | CD8a-AA347-363-KRN-3A-GFP | Expresses CD8a with of Arl13b ciliary targeting sequence (CTS) from amino acids 347-363 with KRN-3A mutation fused to the cytosolic tail and GFP-tagged |
| 221599 | CD8a-AA354-363-GFP | Expresses CD8a fused with a segment of the Arl13b protein (amino acids 354-363) and GFP-tagged |
| 247082 | AA1-245-GFP | Expresses mammalian Arl13b with amino acids 1-245 and GFP-tagged |
| 247240 | His-AA1-245-GFP | A bacterial expression plasmid encoding Arl13b (aa1-245) with N-terminal His and GFP-tagged |
| 247241 | His-AA195-428-GFP | A bacterial expression plasmid encoding Arl13b (aa195-428) with N-terminal His and GFP-tagged |
| 247242 | His-GFP | A bacterial expression plasmid encoding GFP with N-terminal His |
| 247243 | His-13bCTS-GFP | A bacterial expression plasmid encoding ciliary targeting sequence of mammalian Arl13b (amino acids 347-363) with N-terminal His and GFP-tagged |
| 247244 | His-MBP-GFP | A bacterial expression plasmid encoding maltose binding protein with N-terminal His and GFP-tagged |
| 247762 | CD8a-AA354-361-GFP | Expresses CD8a fused with a segment of the Arl13b protein (amino acids 354-361) and GFP-tagged |
| 247767 | CD8a-AA358-361-GFP | Expresses CD8a fused with a segment of the Arl13b protein (amino acids 358-361) and GFP-tagged |