Christopher A. O'Callaghan Lab: UniClo: Scarless hierarchical DNA assembly without sequence constraint
Flores N, O’Callaghan C
Nucleic Acids Research
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 221559 | POC1423 | Plasmid designed to assay the site-selective methylation protection approach using the non-switchable methylases |
| 221568 | POC1343 | Plasmid containing the Insert 1 to be assembled in POC1355 |
| 221569 | POC1344 | Plasmid containing the Insert 2 to be assembled in POC1355 |
| 221570 | POC1345 | Plasmid containing the Insert 3 to be assembled in POC1355 |
| 221571 | POC1346 | Plasmid containing the Insert 4 to be assembled in POC1355 |
| 221572 | POC1355 | Assembly vector designed to be methylated by the M.Osp807II BsaI-associated switch methylase and for the assembly of inserts 1, 2, 3 and 4 from POC1343, POC1344, POC1345 and POC1346; respectively |
| 221573 | POC1426 | Plasmid containing the Insert 1 to be assembled in POC1430 using the site-selective methylation protection approach |
| 221574 | POC1427 | Plasmid containing the Insert 2 to be assembled in POC1430 using the site-selective methylation protection approach |
| 221575 | POC1428 | Plasmid containing the Insert 3 to be assembled in POC1430 using the site-selective methylation protection approach |
| 221576 | POC1429 | Plasmid containing the Insert 4 to be assembled in POC1430 using the site-selective methylation protection approach |
| 221577 | POC1430 | Assembly vector designed to be methylated by the M.Osp807II BsaI-associated switch methylase and for the assembly of inserts 1, 2, 3 and 4 from POC1426, POC1427, POC1428 and POC1429; respectively |
| 221578 | POC1431 | Assembled plasmid containing four inserts (from POC1426, POC1427, POC1428 and POC1429) assembled using the site-selective methylation protection approach |
| 221579 | POC1518 | pMOBK360_VL: Vector Left (VL) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach |
| 221580 | POC1519 | pMOBK360_VM: Vector Middle (VM) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach |
| 221581 | POC1520 | pMOBK360_VR: Vector Right (VR) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach |
| 226476 | POC1535 | pMOLC360_VL_I1 (Chloramphenicol R) containing the insert I1 to be assembled in POC1518 (pMOBK360_VL) using the site-selective methylation protection approach |
| 226477 | POC1536 | pMOLC360_VM_I2 (Chloramphenicol R) containing the insert I2 to be assembled in POC1518 (pMOBK360_VL) using the site-selective methylation protection |
| 226478 | POC1537 | pMOLC360_VM_I3 (Chloramphenicol R) containing the insert I3 to be assembled in POC1518 (pMOBK360_VL) using the site-selective methylation protection |
| 226479 | POC1538 | pMOLC360_VR_I4 (Chloramphenicol R) containing the insert I4 to be assembled in POC1518 (pMOBK360_VL) using the site-selective methylation protection |
| 226480 | POC1539 | pMOLC360_VL_I5 (Chloramphenicol R) containing the insert I5 to be assembled in POC1519 (pMOBK360_VM) using the site-selective methylation protection |
| 226481 | POC1540 | pMOLC360_VM_I6 (Chloramphenicol R) containing the insert I6 to be assembled in POC1519 (pMOBK360_VM) using the site-selective methylation protection |
| 226482 | POC1541 | pMOLC360_VR_I7 (Chloramphenicol R) containing the insert I7 to be assembled in POC1519 (pMOBK360_VM) using the site-selective methylation protection |
| 226483 | POC1542 | pMOLC360_VL_I8 (Chloramphenicol R) containing the insert I8 to be assembled in POC1519 (pMOBK360_VM) using the site-selective methylation protection |
| 226484 | POC1543 | pMOLC360_VR_I9 (Chloramphenicol R) containing the insert I9 to be assembled in POC1519 (pMOBK360_VM) using the site-selective methylation protection |
| 226485 | POC1544 | pMOLC360_VL_I10 (Chloramphenicol R) containing the insert I10 to be assembled in POC1520 (pMOBK360_VR) using the site-selective methylation protection |
| 226486 | POC1545 | pMOLC360_VR_I11 (Chloramphenicol R) containing the insert I11 to be assembled in POC1520 (pMOBK360_VR) using the site-selective methylation protection |
| 226487 | POC1546 | pMOBK360_VL_N1 assembled plasmid (Kanamycin R) containing four inserts (POC1535-POC1538) assembled in POC1518 using the site-selective methylation protection approach |
| 226489 | POC1547 | pMOBK360_VM_N2 assembled plasmid (Kanamycin R) containing three inserts (POC1539-POC1541) assembled in POC1519 using the site-selective methylation protection approach |
| 226490 | POC1548 | pMOBK360_VM_N3 assembled plasmid (Kanamycin R) containing two inserts (POC1542-POC1543) assembled in POC1519 using the site-selective methylation protection approach |
| 226491 | POC1549 | pMOBK360_VR_N4 assembled plasmid (Kanamycin R) containing two inserts (POC1544-POC1545) assembled in POC1520 using the site-selective methylation protection approach |
| 226496 | POC1525 | pMOBC360_VM: Vector Middle (VM) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach |
| 226497 | POC1550 | pMOBC360_VM_C1 assembled plasmid (Chloramphenicol R) containing four inserts (POC1546-POC1549) assembled in POC1525 using the site-selective methylation protection approach |
| 226498 | POC1551 | pMOBK360_StandVKL: Standard Vector (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection |
| 226499 | POC1552 | pMOBC360_StandVC: Standard Vector (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection |
| 236103 | POC1358 | Assembled plasmid containing four inserts (from POC1343, POC1344, POC1345 and POC1346) assembled in POC1355 |