High throughput variant libraries and machine learning yield design rules for retron gene editors.
Crawford KD, Khan AG, Lopez SC, Goodarzi H, Shipman SL
Nucleic Acids Res. 2025 Jan 11;53(2):gkae1199. doi: 10.1093/nar/gkae1199.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222223 | pAGK43 | Edits NPAS2 Gene. |
| 222224 | pKDC.100 | Acceptor plasmid for yeast retron-Eco1 ncRNA variant libraries. |
| 222225 | pSCL194 | Expresses site 1/Cas9/Eco1 RT in HIS3 locus. |
| 222226 | pSCL195 | Expresses site 2/Cas9/Eco1 RT in HIS3 locus. |
| 222227 | pSCL368 | Expresses site 3/Cas9/Eco1 RT in HIS3 locus. |
| 222228 | pKDC.154 | Acceptor plasmid for human retron-Eco1 variants. |