KIF1C activates and extends dynein movement through the FHF cargo adapter.
Abid Ali F, Zwetsloot AJ, Stone CE, Morgan TE, Wademan RF, Carter AP, Straube A
Nat Struct Mol Biol. 2025 Apr;32(4):756-766. doi: 10.1038/s41594-024-01418-z. Epub 2025 Jan 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222287 | pFastBac-M13-8xHis-ZZ-LTLT-HOOK3 | Expression of HOOK3 in insect cells for purification of untagged adapter (8xHIS-ZZtag can be cleaved off with TEV protease). |
| 222288 | pET22b-GST-6P2-LTLT-KIF1C(642–922)-GFP-6His | Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal GFP and His tag in E.coli for purification. |
| 222289 | pET22b-GST-6P2-LTLT-KIF1C(642–922)-6His | Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal 6xHis tag in E.coli for purification. |
| 222299 | pBIG1a_StrepII-Hook3_FTS_FHIP1B | Co-expresses full-length human FHF (FTS, StrepII-HOOK3 and FHIP1B) in a pBIG1a vector |
| 222300 | pBIG1a_StrepII-Hook3[451-718]_FTS_FHIP1B | Co-expresses truncated human FHF (FTS, StrepII-HOOK3[451-718] and FHIP1B) in a pBIG1a vector |
| 222301 | pBIG1a_StrepII-Hook3[571-718]_FTS_FHIP1B | Co-expresses truncated human FHF (FTS, StrepII-HOOK3[571-718] and FHIP1B) in a pBIG1a vector |
| 222302 | pBIG1a_StrepII-Hook2_FTS_FHIP2A | Co-expresses full-length human FHF (FTS, StrepII-HOOK2 and FHIP2A) in a pBIG1a vector |