Development of a tightly regulated copper-inducible transient gene expression system in Nicotiana benthamiana incorporating a suicide exon and Cre recombinase.
Chiang BJ, Lin KY, Chen YF, Huang CY, Goh FJ, Huang LT, Chen LH, Wu CH
New Phytol. 2024 Oct;244(1):318-331. doi: 10.1111/nph.20021. Epub 2024 Jul 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222435 | pCHW00010 | Level 0, promoter module (GGAG-AATG) containing CBS4 and tomato miniDFR promoters. |
| 222436 | pCHW90004 | Level 0, promoter module (GGAG-CCAT) containing CBS4 and tomato miniDFR promoters. |
| 222437 | pCHW110031 | Level 0, promoter module (GGAG-CCAT) containing CBS4 and CMV1 promoters. |
| 222438 | pCHW110032 | Level 0, promoter module (GGAG-AATG) containing CBS4 and CMV1 promoters. |
| 222439 | pCHW00011 | Level 0, CDSns module (AATG-TTCG) containing S. cerevisiae CUP2. |
| 222440 | pCHW100002 | Level 0, CDS module (AATG-GCTT) containing rice OsL5. |
| 222441 | pCHW110033 | Level 0, CDS module (AATG-GCTT) containing Cre recombinase. |
| 222442 | pCHW100003 | Level 0, N-terminal tag module (CCAT-AATG) containing mCherry2-HyP5SM. |
| 222443 | pCHW110034 | Level 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry. |
| 222444 | pCHW110035 | Level 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry-stop. |
| 222445 | pCHW00014 | Level 0, C terminal tag module (TTCG-GCTT) containing VP16-NLS. |
| 222446 | pCHW00015 | Level 0, C terminal tag module (TTCG-GCTT) containig VP64-NLS. |
| 222447 | pCHW00016 | Level 0, C terminal tag module (TTCG-GCTT) containing p65AD-NLS. |
| 222448 | pCHW00019 | Level 0, C terminal tag module (TTCG-GCTT) containing GAL4AD-NLS. |
| 222449 | pCHW91003 | Level 1, Position 3 containing 35S::RUBY::OCS. |
| 222450 | pCHW91013 | Level 1, Position 3 containing CBS4-miniDFR::RUBY::OCS. |
| 222451 | pCHW111040 | Level 1, Position 3 containing CBS4-CMV::RUBY::OCS. |
| 222452 | pCHW111101 | Level 1, Position 7 containing CBS4-miniDFR::Cre::NOS. |
| 222453 | pCHW101008 | Level 1, Position 5 containing CBS4-miniDFR::OsL5::NOS. |
| 222454 | pCHW101020 | Level 1, Position 2 containing 35S::CUP2-p65::NOS. |
| 222455 | pCHW91015 | Level 1, Position 2 containing Mas::CUP2-VP16::NOS. |
| 222456 | pCHW111111 | Level 1 acceptor, Position 3 containing CBS4-miniDFR::LacZ::nosT. |
| 222457 | pCHW111112 | Level 1 acceptor, Position 3 containing CBS4-miniDFR::LacZ::nosT. |
| 222458 | pCHW111113 | Level 1 acceptor, Position 3 containing CBS4-CMV1::LacZ::nosT. |
| 222459 | pCHW111114 | Level 1 acceptor, Position 3 containing CBS4-CMV1::LacZ::nosT. |
| 222460 | pCHW111117 | Level 1 acceptor, Position 3 containing CBS4-CMV1::LoxP-mCherry-stop-LacZ::nosT. |
| 222461 | pCHW111118 | Level 1 acceptor, Position 3 containing CBS4-CMV1::LoxP-mCherry-stop-LacZ::nosT. |
| 222462 | pCHW91069 | Level 1 acceptor, Position 3 containing CBS4-miniDFR::mCherry2-HyP5SM-LacZ::nosT. |
| 222463 | pCHW91070 | Level 1 acceptor, Position 3 containing CBS4-CMV1::mCherry2-HyP5SM-LacZ::nosT. |