The drug-elicitable alternative splicing module for tunable vector expression in the heart.
Chen Z, Yang L, Zhang Y, Li J, Yang Y, Li Y, Fan L, Chen W, Miao L, Liu J, Chen G, Wang Z, Li Y, Gao F, Zhou J, Zheng L, Zhang Y, Zhao D, Pu WT, Yang K, Dong E, Guo Y
Nat Cardiovasc Res. 2025 Jun 13. doi: 10.1038/s44161-025-00665-7.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 223181 | pAAV-CMV-MCS-noATG-GFP | A multicloning site (MCS) was synthesized and inserted into AAV-CMV-GFP to construct the AAV-CMV-MCS-(no ATG)-GFP negative control plasmid. |
| 223184 | pAAV-TNT4-Zfr-P2A-HA-luciferase-3xMIR122 | Plasmid containing the Zfr/Firefly luciferase/3xMIR122 cassette. Translation of luciferase is controlled by splicing modulation of the Zfr specifically in cardiomyocytes by risdiplam in vivo. |
| 223186 | pAAV-TNT4-Zfr-P2A-HA-YAP5SA-3xMIR122 | Plasmid containing the Zfr/YAP5SA/3xMIR122 cassette. Translation of YAP5SA is controlled by splicing modulation of the Zfr specifically in cardiomyocytes by risdiplam in vivo. |
| 223187 | pAAV-CMV-Zf-GFP | Plasmid containing the Zfr/eGFP cassette. Translation of GFP is controlled by splicing modulation of the Zfr by risdiplam. |
| 223188 | pAAV-TNT4-Zfr-P2A-HA-luciferase | Plasmid containing the Zfr/Firefly luciferase cassette. Translation of luciferase is controlled by splicing modulation of the Zfr by risdiplam. |
| 223189 | pAAV-TNT4-Zfr-P2A-HA-YAP5SA | Plasmid containing the Zfr/YAP5SA cassette. Translation of YAP5SA is controlled by splicing modulation of the Zfr by risdiplam. |