Rapid and reversible dissolution of biomolecular condensates using light-controlled recruitment of a solubility tag.
Brumbaugh-Reed EH, Gao Y, Aoki K, Toettcher JE
Nat Commun. 2024 Aug 7;15(1):6717. doi: 10.1038/s41467-024-50858-0.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 223285 | pHR myc-FUS-FUS-FusionRed-FUS-FRB-iLID | A phase separating fluorescent protein with optogenetic and chemogenetic tags for recruitment of desired cargo domains. Used to demonstrate condensate dissolution using the OptoMBP system. |
| 223286 | pHR myc-MBP-SspB-iRFP-NLS | An SspB-tagged maltose binding protein (MBP) with an infrared fluorescent protein for visualization. Used to demonstrate condensate dissolution using the OptoMBP system. |
| 223287 | pPB-TetOn-FUS-CHOP-FusionRed-FRB-iLID-WPRE-SFFV-MBP-SspB-irFP-NLS-WPRE | A complete OptoMBP cassette for dissolution of FUS-CHOP condensates. |
| 223288 | pHR hEF1A-BFP-P2A-rtTA-WPRE | A lentiviral vector expressing BFP and the TetON transcription factor rtTA. |
| 223289 | pHR Myc-Oskar-FusionRed-FRB-iLID | The Oskar germ granule protein tagged with optogenetic and chemogenetic tags for recruitment of desired cargo. Used to demonstrate condensate dissolution using the OptoMBP system. |