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Engineering of photo-inducible binary interaction tools for biomedical applications.

Lee YT, Guo L, Lan TH, Nonomura T, Liu G, Ma G, Wang R, Huang Y, Zhou Y
Nat Commun. 2025 Jul 28;16(1):6940. doi: 10.1038/s41467-025-61710-4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
223688Ntom-Venus-ssrAPhoBIT1 component; Venus-tagged ssrA tag fused with Ntom for mitochondrial tethering
223689mCh-sspB(LOV2)PhoBIT1 component; mCherry-tagged sspB with LOV2 insertion
223690STIM1ct-ssrA2-mCeruleanPhoBIT2 component; ssrA2 tag (ssrA mutant A2C) inserted into mCerulean-tagged STIM1 cytoplasmic domain
223691mCh-CRY2-sspB2PhoBIT2 component; sspB2 (sspB mutant A56F) fused to mCh-CRY2PHR

Antibodies from Article