Biochemical reconstitution of a type I-B CRISPR-associated transposon.
Wang S, Chang L
Methods Enzymol. 2025;712:55-79. doi: 10.1016/bs.mie.2025.01.042. Epub 2025 Feb 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 224926 | pCascade_SPS1(PmcCAST) | Expression and purification of type I-B2 Cascade in CRISPR-associated transposon of Romsetta |
| 224927 | pET_StrepII_TEV_LIC_TniQ(PmcCAST) | TniQ expression plasmid for the co-purification of TnsC-TniQ complex |
| 224928 | pR6K-Donor(PmcCAST)_KanR | Transposition assay |