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Establishing a straightforward I-SceI-mediated recombination one-plasmid system for efficient genome editing in P. putida KT2440.

Meng H, Kobbing S, Blank LM
Microb Biotechnol. 2024 Jul;17(7):e14531. doi: 10.1111/1751-7915.14531. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
225132pEMG-RISFor genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Km.
225133pEMG-AISFor genome editing in P. putida KT2440, arabinose is used for the induction of I-sceI gene. Km.
225134pEMG-GRISFor genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Gm.

Antibodies from Article