Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary Polyphenols.
Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 10.1021/jacs.4c09892. Epub 2025 Feb 24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 225296 | pXD80ENSF1-C1858_02810-(–)-Eldh2-Accs (ElA2) | Expresses Eggerthella lenta A2 (–)-Eldh2 in Gordonibacter urolithinfaciens DSM 27213 |
| 225298 | pXD80ENSF3-Elen0507-Cadh-Accs(ElA2) | Expresses Eggerthella lenta A2 Cadh in Gordonibacter urolithinfaciens DSM 27213 |
| 225300 | pXD80ENSF5-Elen0515-eCadh-Accs(ElA2) | Expresses Eggerthella lenta A2 eCadh in Gordonibacter urolithinfaciens DSM 27213 |
| 225301 | pXD80ENSF6-Elen0497-ElVadh-Accs(ElDSM2243) | Expresses Eggerthella lenta DSM 2243 El Vadh in Gordonibacter urolithinfaciens DSM 27213 |
| 225302 | pXD80ENSF7-DadhA(cHis8)BC-DadRS-Accs(ElA2) | Expresses Eggerthella lenta A2 Dadh in Gordonibacter urolithinfaciens DSM 27213 |
| 225303 | pXD80ENSF8-HcdhA(cHis8)BC-HcdR-Accs(ElA2) | Expresses Eggerthella lenta A2 Hcdh in Gordonibacter urolithinfaciens DSM 27213 |
| 225304 | pXD80ENSF18-C1878_02750_GsVadh(Gs28C) | Expresses Gordonibacter sp. 28C Gs Vadh in Gordonibacter urolithinfaciens DSM 27213 |
| 225305 | pXD70EN19-Pct5-GPA_25810/25800-(+)-Eldh1/2(GpDSM19378) | Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 and (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoter |
| 225306 | pXD70EN20-Pct5-GPA_25810-(+)-Eldh1(GpDSM19378) | Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 in Eggerthella lenta DSM 2243 with a cumate inducible promoter |
| 225307 | pXD70EN21-Pct5-GPA_25800-(+)-Eldh2(GpDSM19378) | Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoter |
| 225308 | pXD80EN13-Elen0616-Cber(ElDSM2243) | Expresses Eggerthella lenta DSM 2243 Cber in Gordonibacter urolithinfaciens DSM 27213 |
| 225309 | pXD80EN1-C1858_02810-(–)-Eldh2-noAccs (ElA2) | Expresses Eggerthella lenta A2 (–)-Eldh2 without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213 |
| 225310 | pXD80EN2-Elen0501-(+)-Eddh-noAccs(ElA2) | Expresses Eggerthella lenta A2 (+)-Eddh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213 |
| 225311 | pXD80EN3-Elen0507-Cadh-noAccs(ElA2) | Expresses Eggerthella lenta A2 Cadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213 |
| 225312 | pXD80EN4-Elen0511-(–)-Eldh1-noAccs(ElA2) | Expresses Eggerthella lenta A2 (–)-Eldh1 without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213 |
| 225313 | pXD80EN5-Elen0515-eCadh-noAccs(ElA2) | Expresses Eggerthella lenta A2 eCadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213 |
| 225314 | pXD80EN6-Elen0497-ElVadh-noAccs(ElDSM2243) | Expresses Eggerthella lenta DSM 2243 El Vadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213 |
| 225315 | pXD80ENSF7.1-Dadh-ΔElen0469(ElA2) | Expresses Eggerthella lenta A2 Dadh (ΔElen_0469) in Gordonibacter urolithinfaciens DSM 27213 |
| 225317 | pXD80ENSF7.2-Dadh-ΔElen0470(ElA2) | Expresses Eggerthella lenta A2 Dadh (ΔElen_0470) in Gordonibacter urolithinfaciens DSM 27213 |
| 225318 | pXD80ENSF7.3-Dadh-ΔElen0473(ElA2) | Expresses Eggerthella lenta A2 Dadh (ΔElen_0473) in Gordonibacter urolithinfaciens DSM 27213 |
| 225319 | pXD80ENSF7.4-Dadh-ΔElen0474(ElA2) | Expresses Eggerthella lenta A2 Dadh (ΔElen_0474) in Gordonibacter urolithinfaciens DSM 27213 |
| 225321 | pXD80ENSF7.6-Dadh-ΔElen0476(ElA2) | Expresses Eggerthella lenta A2 Dadh (ΔElen_0476) in Gordonibacter urolithinfaciens DSM 27213 |
| 225322 | pXD80ENSF7.7-Dadh-ΔElen0477(ElA2) | Expresses Eggerthella lenta A2 Dadh (ΔElen_0477) in Gordonibacter urolithinfaciens DSM 27213 |
| 225323 | pXD80ENSF7.8-Dadh-ΔElen0478(ElA2) | Expresses Eggerthella lenta A2 Dadh (ΔElen_0478) in Gordonibacter urolithinfaciens DSM 27213 |
| 225324 | pXD80ENSF7.9-Dadh-ΔElen0481(ElA2) | Expresses Eggerthella lenta A2 Dadh (ΔElen_0481) in Gordonibacter urolithinfaciens DSM 27213 |
| 225325 | pXD80ENSF7.10-Dadh-ΔElen0482(ElA2) | Expresses Eggerthella lenta A2 Dadh (ΔElen_0482) in Gordonibacter urolithinfaciens DSM 27213 |
| 225326 | pXD70DA8-DadhA(cHis8)BC-DadRS-noAccs(ElA2) | Expresses Eggerthella lenta A2 Dadh in Eggerthella lenta DSM 2243 |
| 225328 | pXD80EN16-HcdhA(cHis8)BC-partial cluster(ElA2) | Expresses Eggerthella lenta A2 Hcdh partial gene cluster (inactive) in Gordonibacter urolithinfaciens DSM 27213 |
| 225329 | pXD80RFP-pXD68Kan2-RFP | RSF1010-based broad host-range plasmid engineered from pXD68Kan2 encoding RFP, kanamycin resistance |
| 233868 | pXD80ENSF9-Pct3-(–)-Eldh2(cHis6)-Accs(ElA2) | Expresses His-tagged Eggerthella lenta A2 (–)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification |
| 233869 | pXD80ENSF10-Pct3-(+)-Eddh(cHis6)-Accs(ElA2) | Expresses His-tagged Eggerthella lenta A2 (+)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification |
| 233870 | pXD80ENSF12-Pct3-(–)-Eldh1(cHis6)-Accs(ElA2) | Expresses His-tagged Eggerthella lenta A2 (–)-Eldh1 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification |
| 233871 | pXD80ENSF14-Pct3-ElVadh(cHis6)-Accs(ElDSM2243) | Expresses His-tagged Eggerthella lenta DSM 2243 El Vadh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification |
| 233873 | pXD80ENSF25-Pct3-(–)-Eddh(nHis6)(G3C) | Expresses His-tagged Gordonibacter pamelaeae 3C (–)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification |
| 233874 | pXD80ENSF26-Pct3-(+)-Eldh1(nHis6)(GpDSM19378) | Expresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification |
| 233875 | pXD80ENSF27-Pct3-(+)-Eldh2(nHis6)(GpDSM19378) | Expresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification |
| 233876 | pXD80ENSF30-DadhABC(cHis8)RS-Accs(Cadh)(ElA2) | Expresses Eggerthella lenta A2 Dadh with the accessory genes from the cadh gene cluster in Gordonibacter urolithinfaciens DSM 27213 |
| 233877 | pXD70EN25-Pct5-(–)-Eddh(Gp3C) | Expresses Gordonibacter pamelaeae 3C (–)-Eddh in Eggerthella lenta DSM 2243 with a cumate inducible promoter |
| 233879 | pXD80EN8-Pct3-(+)-Eddh(cHis6)-noAccs(ElA2) | Expresses His-tagged Eggerthella lenta A2 (+)-Eddh with a cumate inducible promoter, without accessory genes (inactive), in Gordonibacter urolithinfaciens DSM 27213 |
| 233880 | pXD80EN10-Pct3-(–)-Eldh1(cHis6)-noAccs(ElA2) | Expresses His-tagged Eggerthella lenta A2 (–)-Eldh1 with a cumate inducible promoter, without accessory genes (inactive), in Gordonibacter urolithinfaciens DSM 27213 |