SelectRepair Knockout: Efficient PTC-Free Gene Knockout Through Selectable Homology-Directed DNA Repair.
Cortazar MA, Jagannathan S
Methods Mol Biol. 2025;2863:397-417. doi: 10.1007/978-1-0716-4176-7_23.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 225357 | LIG4 HAL-T2A-GFP-SV40pA-HAR | This plasmid serves as a template for homologous DNA recombination at the LIG4 gene to knock out the DNA ligase 4 protein using the SelectRepair knockout GFP approach. |
| 225358 | LIG4 HAL-T2A-HYG-SV40pA-HAR | This plasmid serves as a template for homologous DNA recombination at the LIG4 gene to knock out the DNA ligase 4 protein using the hygromycin B SelectRepair knockout approach. |
| 225359 | LIG4 HAL-T2A-NEO-SV40pA-HAR | This plasmid serves as a template for homologous DNA recombination at the LIG4 gene to knock out the DNA ligase 4 protein using the neomycin SelectRepair knockout approach. |
| 225363 | pX459_LIG4_Exon3 | pX459 plasmid encoding the sgRNA protospacer sequence “5’-GCATAATGTCACTACAGATC-3’ to target the LIG4 gene. |