Positive selection CRISPR screens reveal a druggable pocket in an oligosaccharyltransferase required for inflammatory signaling to NF-kappaB.
Lampson BL, Ramiotarez AS, Baro M, He L, Hegde M, Koduri V, Pfaff JL, Hanna RE, Kowal J, Shirole NH, He Y, Doench JG, Contessa JN, Locher KP, Kaelin WG Jr
Cell. 2024 Apr 25;187(9):2209-2223.e16. doi: 10.1016/j.cell.2024.03.022.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 246904 | UBC-CCDC134-HA-hygro | Expresses HA-tagged human CCDC134 in mammalian cells |
| 246905 | UBC-TLR4-HA hygro | Expresses HA-tagged human TLR4 in mammalian cells |
| 246906 | CMV-CD16-TLR4-HA | Expresses CD16-TLR4 chimera in mammalian cells |
| 246908 | STT3A E266K | Expresses an NGI-resistant version of STT3A, STT3A E266K, in mammalian cells |
| 246909 | STT3A F256P | Expresses an NGI-resistant version of STT3A, STT3A F256P, in mammalian cells |
| 246911 | STT3A | Expresses STT3A |
| 246912 | ER-GFP pLVX CMVpuro | Lentiviral-based expression of EGFP with an ER retention signal sequence in mammalian cells |
| 246913 | ER-GFP Q185N N186C pLVX CMVpuro | Lentiviral-based expression of EGFP with an ER retention signal sequence and Q185N N186C, an STT3B-specific glcosylation sequon |
| 246914 | NFKB3-CK-IRES-GFP hygro | NFKB-dependent expression of a bicistronic cDNA encoding a suicide gene (modified deoxycytidine kinase) and EGFP |