Thalamocortical organoids enable in vitro modeling of 22q11.2 microdeletion associated with neuropsychiatric disorders.
Shin D, Kim CN, Ross J, Hennick KM, Wu SR, Paranjape N, Leonard R, Wang JC, Keefe MG, Pavlovic BJ, Donohue KC, Moreau C, Wigdor EM, Larson HH, Allen DE, Cadwell CR, Bhaduri A, Popova G, Bearden CE, Pollen AA, Jacquemont S, Sanders SJ, Haussler D, Wiita AP, Frost NA, Sohal VS, Nowakowski TJ
Cell Stem Cell. 2024 Mar 7;31(3):421-432.e8. doi: 10.1016/j.stem.2024.01.010. Epub 2024 Feb 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 231018 | pSicoFlipped-CAG-GFP-WPRE-bGHpA | For strong lentivirus-mediated expression of GFP |
| 231019 | pSicoFlipped-CAG-dTomato-WPRE-bGHpA | For strong lentivirus-mediated expression of dTomato |
| 231020 | pSicoFlipped-CAG-GFP-2A-FOXP2-WPRE-bGHpA | For lentivirus-mediated co-expression of GFP and FOXP2 |
| 231021 | pSicoFlipped-CAG-GFP-2A-DGCR8-WPRE-bGHpA | For lentivirus-mediated co-expression of GFP and DGCR8 |
| 231022 | pLV-hU6-FOXP2_sgRNA1-hUbC-dCas9-KRAB-T2a-GFP | All-in-one lentiviral vector (Gersbach lab design) for CRISPRi targeting FOXP2 |
| 231023 | pLV-hU6-FOXP2_sgRNA2-hUbC-dCas9-KRAB-T2a-GFP | All-in-one lentiviral vector (Gersbach lab design) for CRISPRi targeting FOXP2 |
| 231024 | pLV-hU6-ROBO2_sgRNA-hUbC-dCas9-KRAB-T2a-GFP | All-in-one lentiviral vector (Gersbach lab design) for CRISPRi targeting ROBO2 |
| 231025 | pLV-hU6-NTC_sgRNA-hUbC-dCas9-KRAB-T2a-GFP | All-in-one lentiviral vector (Gersbach lab design) for CRISPRi with non-targeting control sgRNA |