IntAct: A nondisruptive internal tagging strategy to study the organization and function of actin isoforms.
van Zwam MC, Dhar A, Bosman W, van Straaten W, Weijers S, Seta E, Joosten B, van Haren J, Palani S, van den Dries K
PLoS Biol. 2024 Mar 11;22(3):e3002551. doi: 10.1371/journal.pbio.3002551. eCollection 2024 Mar.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 250439 | pcDNA3.1(+)_BetaActin_ALFA_229230 | Expresses tagged beta cytoplasmic actin in mammalian cells. Protein is tagged with an internal ALFA tag at position T229/A230. |
| 250440 | pcDNA3.1(+)_GammaActin_ALFA_229230 | Expresses tagged gamma cytoplasmic actin in mammalian cells. Protein is tagged with an internal ALFA tag at position T229/A230. |
| 250441 | pcDNA3.1(+)_AlphaSMA_ALFA_231232 | Expresses tagged alpha smooth muscle actin in mammalian cells. Protein is tagged with an internal ALFA tag at position T231/A232. |
| 250442 | pcDNA3.1(+)_GammaSMA_ALFA_230231 | Expresses tagged gamma smooth muscle actin in mammalian cells. Protein is tagged with an internal ALFA tag at position T230/A231. |
| 250443 | pcDNA3.1(+)_AlphaCARD_ALFA_231232 | Expresses tagged alpha cardiac muscle actin in mammalian cells. Protein is tagged with an internal ALFA tag at position T231/A232. |
| 250444 | pcDNA3.1(+)_AlphaSKE_ALFA_231232 | Expresses tagged alpha skeletal muscle actin in mammalian cells. Protein is tagged with an internal ALFA tag at position T231/A232. |