Engineering a synthetic gene circuit for high-performance inducible expression in mammalian systems.

De Carluccio G, Fusco V, di Bernardo D
Nat Commun. 2024 Apr 17;15(1):3311. doi: 10.1038/s41467-024-47592-y. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
242433	pCMV-TO-CasRx(NLS)-T2A-mCherry	CasRx (NLS-RfxCas13d-NLS) for RNA targeting under the CMV-TO promoter (CMV with two Tet-Operators)
242434	pTRE3G-Fluc-DR	Firefly Luciferse with a Direct Repeat sequence in the 3’UTR recognised by the CasRx under the control of the TRE3G promoter.
242435	pCMV-gLuc-DR_pCMV-RedFireFly.gb	Gaussia Luciferse with a Direct Repeat sequence in the 3’UTR recognised by the CasRx under the control of the CMV promoter, and a RedFirefly Luciferase driven by a CMV promoter.