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Recording morphogen signals reveals mechanisms underlying gastruloid symmetry breaking.

McNamara HM, Solley SC, Adamson B, Chan MM, Toettcher JE
Nat Cell Biol. 2024 Oct 2. doi: 10.1038/s41556-024-01521-9. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
225522pHM028Encodes constitutive expression of mCherry
225523pHM039Encodes Wnt-responsive rtTA and constitutive BFP
225524pHM066Encodes Wnt-responsive destablized iRFP
225525pHM093Encodes rtTA under IBRE4 promoter, and constitutive BFP
225526pHM092Encodes rtTA under AR8 promoter, and constitutive BFP
225527pHM103Encodes constitutive GFP
225528pHM126Encodes Wnt-responsive destablized GFP
225529pHM130Encodes destablized GFP under IBRE4 promoter, and constitutive BFP
225530pHM131Encodes destablized mCherry under IBRE4 promoter, and constitutive BFP
225532pHM148Encodes constitutive rtTA, tetOn-Pcdh19-GFP, and constitutive mCherry
225533H2B-iRFPEncodes H2B-iRFP
225534pHM129Encodes destablized mCherry under AR8 promoter, and constitutive BFP

Antibodies from Article