Absolute Quantification of Mammalian MicroRNAs for Therapeutic RNA Cleavage and Detargeting.
Kraus C, Wang J, Zheng H, Broderick J, Ajaykumar N, Zamani M, Yang M, Cecchini K, Liang SQ, Kolumba O, Chase K, Lee J, Xue W, Sontheimer E, Gainetdinov I
RNA. 2025 May 28:rna.080566.125. doi: 10.1261/rna.080566.125.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 226109 | pEJS1350 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-92a CMV-BFP | Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control. |
| 226110 | pEJS1351 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-378a CMV-BFP | Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control. |
| 226111 | pEJS1352 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-22 CMV-BFP | Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control. |
| 226112 | pEJS1353 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-33b CMV-BFP | Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control. |
| 226113 | pEJS1354 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-1 CMV-BFP | Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control. |
| 226114 | pEJS1355 CMV-AcrIIC3-FLAGNLS-mCherry CMV-BFP | Mammalian expression of AcrIIC3-mCherry fusion without microRNA target sites in the 3' UTR as a control reporter of microRNA repression. BFP expressed separately as a transfection control. |
| 226115 | pEJS1386 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-107 CMV-BFP | Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control. |