Plasmid2MC: efficient cell-free generation of high-purity minicircle DNA for genome editing in mammalian cells.

Oliynyk RT, Mahas A, Karpinski E, Church GM
Commun Biol. 2025 Dec 16;8(1):1778. doi: 10.1038/s42003-025-09157-7. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
226108	p-att-ef1a-MS2-RecT-dCas9-BSD	Using ef-1a promoter, expresses dCas9 and RecT protein that intended to be tethered via MS2 gRNA hairpin to dCas9, and Blasticidin selection
226116	p-att-ef1a-ABE8e-dCas9-BSD	Using promoter ef1a expresses ABE8e-dCas9 protein with Blasticidin selection