Plasmid2MC: efficient cell-free generation of high-purity minicircle DNA for genome editing in mammalian cells
Oliynyk RT, Mahas A, Karpinski E, Church GM
Commun Biol 8 (2025). https://doi.org/10.1038/s42003-025-09157-7
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 226108 | p-att-ef1a-MS2-RecT-dCas9-BSD | Using ef-1a promoter, expresses dCas9 and RecT protein that intended to be tethered via MS2 gRNA hairpin to dCas9, and Blasticidin selection |
| 226116 | p-att-ef1a-ABE8e-dCas9-BSD | Using promoter ef1a expresses ABE8e-dCas9 protein with Blasticidin selection |