Improving growth of Cupriavidus necator H16 on formate using adaptive laboratory evolution-informed engineering.

Calvey CH, Sanchez I Nogue V, White AM, Kneucker CM, Woodworth SP, Alt HM, Eckert CA, Johnson CW
Metab Eng. 2023 Jan;75:78-90. doi: 10.1016/j.ymben.2022.10.016. Epub 2022 Nov 9. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
226198	pCHC004 (∆phaCAB)	Electroporation knockout plasmid (pK18sB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.
226199	pCHC005 (∆RE)	Conjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).
226200	pCHC022 (∆phcA)	Electroporation knockout plasmid (pK18sB) to delete the C. necator LysR-type transcriptional regulator PhcA.
226201	pCHC023 (∆MBH)	Electroporation knockout plasmid (pK18sB) to delete the C. necator membrane-bound hydrogenase operon (deltaMBH).
226202	pCHC024 (∆SH)	Electroporation knockout plasmid (pK18sB) to delete the C. necator soluble hydrogenase operon (deltaSH).
226203	pCHC025 (∆CBBp)	Electroporation knockout plasmid (pK18sB) to delete the C. necator megaplasmid copy of the CBB operon (deltaCBBp).
226204	pCHC027 (∆pemK)	Conjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.
226205	pCHC030 (∆CBBp ∆MBH ∆SH)	Electroporation knockout plasmid (pK18sB) to delete the CBBp, membrane-bound hydrogenase, soluble hydrogenase, and intervening sequences (∆CBBp ∆MBH ∆SH).
226206	pCHC036 (∆pHG1)	Conjugation knockout plasmid (pK18msB) to delete the C. necator megaplasmid replication region containing helD, repA, repB, and parAB in order to promote loss of the entire pHG1 megaplasmid.
226207	pCHC039 (∆phcA)	Conjugation knockout plasmid (pK18msB) to delete the C. necator LysR-type transcriptional regulator PhcA.
226208	pCHC042 (∆phaCAB)	Conjugation knockout plasmid (pK18msB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.