Methods for Visualizing and Quantifying Cholesterol Distribution in Mammalian Cells Using Filipin and D4 Probes.
Eichler J, Huver S, Knorr CJ, Wendling C, Kobayashi T, Tomasetto C, Alpy F
Methods Mol Biol. 2025;2888:101-118. doi: 10.1007/978-1-0716-4318-1_8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 226372 | pET28 His6-mEGFP-D4 | Production of recombinant mEGFP-D4, a fluorescent probe binding cholesterol |
| 226373 | pET28 His6-mEGFP-D4H | Production of recombinant mEGFP-D4H, a fluorescent probe binding cholesterol |
| 226404 | pEGFPC1 MOSPD1 | Expression of human MOSPD1 fused to EGFP in mammalian cells |
| 226405 | pEGFPC1 MOSPD3 | Expression of human MOSPD3 fused to EGFP in mammalian cells |