CRISPRi screen identifies FprB as a synergistic target for gallium therapy in Pseudomonas aeruginosa.

Zhang Y, Zhang T, Xiao X, Wang Y, Kawalek A, Ou J, Ren A, Sun W, de Bakker V, Liu Y, Li Y, Yang L, Ye L, Jia N, Veening JW, Liu X
Nat Commun. 2025 Jul 1;16(1):5870. doi: 10.1038/s41467-025-61208-z. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
226434	ptet-lux-Tn7T-Gm	This vector features a tetracycline-inducible system to regulate luxABCDE reporter expression, optimized for efficiency in P. aeruginosa.
234855	Pooled sgRNA Libraries Set 1 for Pseudomonas aeruginosa PA14	A CRISPRi library for inducible, genome-wide gene repression in Pseudomonas aeruginosa PA14.
237425	S17-1λpir gyrAR462C	This engineered E. coli S17-1 λpir strain, featuring a mutation in the gyrA gene (462Arg→Cys), was designed to confer resistance to the CcdB toxin, allowing it to survive with a ccdB-carrying plasmid.