Expanding the molecular grammar of polar residues and arginine in FUS phase separation.
Wake N, Weng SL, Zheng T, Wang SH, Kirilenko V, Mittal J, Fawzi NL
Nat Chem Biol. 2025 Jul;21(7):1076-1088. doi: 10.1038/s41589-024-01828-6. Epub 2025 Feb 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 226596 | FUS LC-RGG1 wild type | Wild type sequence of FUS LC-RGG1 domains |
| 226597 | FUS LC 12S->G | 12 serines to glycines |
| 226598 | FUS LC 12S->A | 12 serines to alanines |
| 226599 | FUS LC 12S->N | 12 serines to asparagines |
| 226600 | FUS LC 4QQ->AA | 4 glutamine pairs to alanine pairs at positions 27, 28, 92, 93, 132, 133, 157, 158 |
| 226601 | FUS LC 4QQ->GG | 4 glutamine pairs to glycine pairs at positions 27, 28, 92, 93, 132, 133, 157, 158 |
| 226602 | FUS LC 4QQ->NN | 4 glutamine pairs to asparagine pairs at positions 27, 28, 92, 93, 132, 133, 157, 158 |
| 226603 | FUS LC 4S->A#1 | 4 serines to alanine at residue positions 30, 44, 48, 53 |
| 226604 | FUS LC 4S->A#2 | 4 serines to alanine at residue positions 70, 84, 89, 95 |
| 226605 | FUS LC 4S->A#3 | 4 serines to alanine at residue positions 108, 117, 119, 127 |
| 226606 | FUS LC 8Y->S | 8 tyrosines to serine |
| 226607 | FUS LC StoG | All serines to glycines |
| 226608 | FUS LC TtoS | All threonines to serine |
| 226609 | FUS LC QtoA | All glutamines to alanine |
| 226610 | FUS LC-RGG1 StoG | All serines in the LC domain to glycine |
| 226611 | FUS LC-RGG1 TtoS | All threonines in the LC domain to serine |
| 226612 | FUS LC-RGG1 QtoA | All glutamines in the LC domain to alanine |
| 226613 | FUS LC-RGG1 TtoV | All threonines in the LC domain to valine |
| 226614 | FUS LC-RGG1 QtoG | All glutamines in the LC domain to glycine |
| 226615 | FUS LC-RGG1 QtoN | All glutamines in the LC domain to asparagine |
| 226616 | FUS LC-RGG1 QtoS | All glutamines in the LC domain to serine |
| 226617 | FUS LC noYQ_noYT | T7S / Q160S double mutation |