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Implementation of RAGATH RNA-associated DNA Endonucleases as Genome Editing Tool in Escherichia coli.

Zhou X, Yang S, Sun B, Dong F, Yin M, Jiang Y, Huang Z, Yang S
Biotechnol J. 2025 Mar;20(3):e70005. doi: 10.1002/biot.70005. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
226820pISFba1Expressing ISEre1 controled by pCas promoter, λ-red recombinase, SacB and ISFba1 guide RNA targeting pMB1 ori
226821pISEre1reRNAExpressing ISEre1 guide RNA targeting maeB site controled by PJ23119
226822pISFba1reRNAExpressing ISFba1 guide RNA targeting maeB site controled by PJ23119

Antibodies from Article