Symbolic recording of signalling and cis-regulatory element activity to DNA.
Chen W, Choi J, Li X, Nathans JF, Martin B, Yang W, Hamazaki N, Qiu C, Lalanne JB, Regalado S, Kim H, Agarwal V, Nichols E, Leith A, Lee C, Shendure J
Nature. 2024 Aug;632(8027):1073-1081. doi: 10.1038/s41586-024-07706-4. Epub 2024 Jul 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 179157 | PB-5'-ENGRAM | piggyBac plasmid for 5' ENGRAM recorder |
| 179158 | PB-3-FT-ENGRAM | piggyBac plasmid for 3'-FT ENGRAM recorder |
| 239972 | P021-mPB-ENGRAM-All-in-one-PGK-BFP-P2A-Blast | Updated ENGRAM plasmid that is ready for both 5' and 3'-FT ENGRAM cloning |
| 239974 | P022-mPB-TRE-PEmax-T2A-mCherry-PGK-rttA-Puro | PiggyBac plasmid for inducible PEmax expression and Puromycin selection |
| 239975 | P023-mPB-synHEK3-BsaI | PiggyBac plasmid for synthetic DNA Tape (HEK3 locus) |
| 239983 | P024-mPB-5xTAPE-BsaI Map | PiggyBac plasmid for synthetic DNA Tape (Typewriter 5X- Tape) |