Organ-specific electrophile responsivity mapping in live C. elegans.
Liu J, Kulkarni A, Gao YQ, Urul DA, Hamelin R, Novotny BA, Long MJC, Aye Y
Cell. 2024 Dec 26;187(26):7450-7469.e29. doi: 10.1016/j.cell.2024.10.014. Epub 2024 Nov 5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 227151 | pCS2+8-cyp-33e1-flag-tev-halo-his | For T-REX experiments of cyp-33e1. A reactive electrophilic species (RES) Sensor. |
| 227152 | pCS2+8-aex-5-flag-tev-halo-his | For T-REX experiments of aex-5. |
| 227153 | pCS2+8-asp-2-flag-tev-halo-his | For T-REX experiments of asp-2. |
| 227154 | pCS2+8-CYP2A6-flag-tev-halo-his | For T-REX experiments of CYP2A6. A reactive electrophilic species (RES) Sensor. |
| 227155 | pCS2+8-PCSK1-flag-tev-halo-his | For T-REX expriments of PCSK1. |
| 227156 | pCS2+8-cyp-33e1(C295A)-flag-tev-halo-his | For T-REX experiments of cyp-33e1 C295A mutant. Less sensing ability to reactive electrophilic species (RES). |
| 227157 | pCS2+8-cyp-33e1(C439A)-flag-tev-halo-his | For T-REX experiments of cyp-33e1 C439A mutant. Less sensing ability to reactive electrophilic species (RES). |
| 227158 | pCS2+8-cyp-33e1(C295A, C439A)-flag-tev-halo-his | For T-REX experiments of cyp-33e1 C295A and C439A double mutant. Almost no sensing ability to reactive electrophilic species (RES). |
| 227159 | pCS2+8-CYP2A6(C14A)-flag-tev-halo-his | For T-REX experiments of CYP2A6 C14A mutant. A reactive electrophilic species (RES) Sensor. |
| 227160 | pCS2+8-CYP2A6(C82A)-flag-tev-halo-his | For T-REX experiments of CYP2A6 C82A mutant. Less sensing ability to reactive electrophilic species (RES). |
| 227161 | pCS2+8-CYP2A6(C439A)-flag-tev-halo-his | For T-REX experiments of CYP2A6 C439A mutant. Less sensing ability to reactive electrophilic species (RES). |
| 227163 | pCS2+8-CYP2A6(C82A, C439A)-flag-tev-halo-his | For T-REX experiments of CYP2A6 C82A and C439A double mutant. Almost no sensing ability to reactive electrophilic species (RES). |
| 227164 | pCS2+8-CYP2A6(C14A, C82A, C439A)-flag-tev-halo-his | For T-REX experiments of CYP2A6 C14A, C82A, and C439A triple mutant. Almost no sensing ability to reactive electrophilic species (RES). |
| 227165 | pCS2+8-CYP2A6-flag-tev-his | For overexpression of CYP2A6 in mammalian cells and purification. |
| 227166 | pCS2+8-CYP2A6(C82A)-flag-tev-his | For overexpression of CYP2A6 C82A mutant in mammalian cells and purification. |
| 227167 | pCS2+8-CYP2A6(C439A)-flag-tev-his | For overexpression of CYP2A6 C439A mutant in mammalian cells and purification. |
| 227169 | pWormgate2-myo-2p-gfp-halo | For organ-specific expression of GFP-Halo in the pharyngeal muscle of C. elegans. |
| 227171 | pWormgate2-myo-3p-gfp-halo | For organ-specific expression of GFP-Halo in the body-wall muscle and neighboring vulval muscle cells of C. elegans. |
| 227172 | pWormgate2-myo-2p-mCherry-P2A-flag-UltraID | For organ-specific expression of separated mCherry and UltraID in the pharyngeal muscle of C. elegans. |
| 227173 | pWormgate2-ges-1p-mCherry-P2A-flag-UltraID | For organ-specific expression of separated mCherry and UltraID in the intestine of C. elegans. |
| 227174 | pWormgate2-myo-3p-mCherry-P2A-flag-UltraID | For organ-specific expression of separated mCherry and UltraID in the body-wall muscle and neighboring vulval muscle cells of C. elegans. |