Fluorescence-based CRISPR interference system for controlled genetic repression and live single-cell imaging in mycobacteria.
Laudouze J, Point V, Achache W, Crauste C, Canaan S, Santucci P
FEBS Lett. 2025 Feb;599(4):488-501. doi: 10.1002/1873-3468.15071. Epub 2024 Dec 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 227428 | pJL31 | Kanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi) |
| 227429 | pJL32 | Kanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato) |
| 227430 | pJL33 | Kanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi) |
| 227431 | pJL34 | Kanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato) |