PD-linked LRRK2 G2019S mutation impairs astrocyte morphology and synapse maintenance via ERM hyperphosphorylation
Wang S, Baumert R, Séjourné G, Bindu DS, Dimond K, Sakers K, Vazquez L, Moore JL, Tan CX, Takano T, Rodriguez MP, Brose N, Bradley L, Lessing R, Soderling SH, La Spada AR, Eroglu C
eLife 2025 [Reviewed Preprint] 14:RP107556. doi: https://doi.org/10.7554/eLife.107556.1
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 227681 | pZac2.1-GfaABC1D-2xEzrin-BioID2-HA | To over express 2x Ezrin under gfaABC1D promoter in astrocytes |
| 227682 | pZac2.1-GfaABC1D-Ezrin T567D-BioID2-HA | To over express Phospho-mimetic Ezrin under gfaABC1D promoter in astrocytes |
| 227683 | pZac2.1-GfaABC1D-Ezrin T567A-BioID2-HA | To over express Phospho-dead Ezrin under gfaABC1D promoter in astrocytes |
| 227684 | pLKO.1-shAtg7-EGFP | Expresses EGFP and shRNA targeting Atg7 |
| 227685 | pLKO.1-shControl-EGFP | Expresses EGFP and scramble of shRNA targeting Atg7 |
| 227686 | pPB-CAG-shLRRK2-mCherry-CAAX | Membrane tethered mCherry and shRNA targeting LRRK2 |
| 227687 | pPB-CAG-shAtg7-mCherry-CAAX | Membrane tethered mCherry and shRNA targeting Atg7 |
| 227689 | pPB-CAG-shControl-mCherry-CAAX | Membrane tethered mCherry and scramble of shRNA targeting Atg7 |