Syn-tasiR-VIGS: virus-based targeted RNAi in plants by synthetic trans-acting small interfering RNAs derived from minimal precursors.

Cisneros AE, Alarcia A, Llorens-Gamez JJ, Puertes A, Juarez-Molina M, Primc A, Carbonell A
Nucleic Acids Res. 2025 Feb 27;53(5):gkaf183. doi: 10.1093/nar/gkaf183. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
227962	pENTR-B/c	Entry plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.
227963	pMDC32B-B/c	Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.
227964	pENTR-AtmiR173aTS-B/c	Entry plasmid with AtmiR173aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.
227965	pMDC32B-AtmiR173aTS-B/c	Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Arabidopsis thaliana.
227966	pENTR-NbmiR482aTS-B/c	Entry plasmid with NbmiR482aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.
227967	pMDC32B-NbmiR482aTS-B/c	Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Nicotiana benthamiana.