Wade Harper Lab: Harper lab Rab11 and inflammasome reporter plasmids

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
228030	pENTR223 Rab11A WT-STOP	DONR vector for shuttling Rab11A WT into DEST vector. Contains a STOP codon.
228031	pENTR223 Rab11A S20V-STOP	DONR vector for shuttling Rab11A S20V (constitutively active mutation) into DEST vector. Contains a STOP codon.
228032	pENTR223 Rab11A S25N-STOP	DONR vector for shuttling Rab11A S25N (Dominant negative mutation) into DEST vector. Contains a STOP codon.
228033	pENTR223 Rab11A Q70L-STOP	DONR vector for shuttling Rab11A Q70L (constitutively active mutation) into DEST vector. Contains a STOP codon.
228034	pDONR223 Rab25-STOP	DONR vector for shuttling Rab25 into DEST vector. Contains a STOP codon.
228035	pHAGE mCherry-Rab11A	Mammalian lentiviral expression of mCherry-labeled Rab11.
228036	pHAGE mCherry-Rab11A S20V	Mammalian lentiviral expression of mCherry-labeled Rab11 S20V (constitutively active mutation).
228037	pHAGE mCherry-Rab11A S25N	Mammalian lentiviral expression of mCherry-labeled Rab11 S25N (dominant negative mutation).
228038	pHAGE mCherry-Rab11A Q70L	Mammalian lentiviral expression of mCherry-labeled Rab11 Q70L (constitutively active mutation).
228039	pHAGE mCherry-Rab25	Mammalian lentiviral expression of mCherry-labeled Rab25.
228040	Rab11A[8-175]-His-C	Protein purification construct. Rab11 crystallography truncation with a C-terminal cys for maleimide labeling.
228042	Rab11A[8-175]-His-C Q70L	Protein purification construct. Rab11 crystallography truncation with a C-terminal cys for maleimide labeling. Constitutively active mutation (Q70L).
228043	pDONR223 CASP1[CARD]-mSG2 (inflammasome reporter)	DONR vector for inflammasome speck reporter, designed from PMC9623368.
228044	pLentiUBC CASP1[CARD]-mSG2 (inflammasome reporter)	Lentiviral stable Inflammasome speck reporter, designed from PMC9623368.