High-throughput optimisation of protein secretion in yeast via an engineered biosensor.

Cleaver A, Luo R, Smith OB, Murphy L, Schwessinger B, Brock J
Trends Biotechnol. 2025 Apr;43(4):838-867. doi: 10.1016/j.tibtech.2024.11.010. Epub 2024 Dec 13. PubMed Article

These materials are included in the MoClo Yeast Secretion Biosensor (YSB) Toolkit.

Plasmids from Article

ID	Plasmid	Purpose
228145	SP_A1	Plasmid contains the secretion signal peptide sequence of the yeast MEL1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228146	SP_A2	Plasmid contains the secretion signal peptide sequence of the yeast (S. uvarum) SPR1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228147	SP_A3	Plasmid contains the secretion signal peptide sequence of the human ECM33 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228148	SP_A4	Plasmid contains the secretion signal peptide sequence of the human CHADL gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228149	SP_A5	Plasmid contains the secretion signal peptide sequence of the human GDF8 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228150	SP_A6	Plasmid contains the secretion signal peptide sequence of the human COLL1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228151	SP_B1	Plasmid contains the secretion signal peptide sequence of the yeast INV1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228152	SP_B2	Plasmid contains the secretion signal peptide sequence of the yeast FIG2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228153	SP_B3	Plasmid contains the secretion signal peptide sequence of the yeast (strain YJM789) PIR1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228154	SP_B4	Plasmid contains the secretion signal peptide sequence of the human LRP8 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228155	SP_B5	Plasmid contains the secretion signal peptide sequence of the human CCL7 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228156	SP_B6	Plasmid contains the secretion signal peptide sequence of the human CREG1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228157	SP_C1	Plasmid contains the secretion signal peptide sequence of the yeast MEL6 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228158	SP_C2	Plasmid contains the secretion signal peptide sequence of the yeast (S. mikatae) MEL gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228159	SP_C3	Plasmid contains the secretion signal peptide sequence of the yeast (strain ATCC 204508 / S288c) PIR1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228160	SP_C4	Plasmid contains the secretion signal peptide sequence of the human AFAM gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228161	SP_C5	Plasmid contains the secretion signal peptide sequence of the human STRCL gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228162	SP_C6	Plasmid contains the secretion signal peptide sequence of the human NELL1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228163	SP_D1	Plasmid contains the secretion signal peptide sequence of the yeast MEL2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228164	SP_D2	Plasmid contains the secretion signal peptide sequence of the yeast DAN1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228165	SP_D3	Plasmid contains the secretion signal peptide sequence of the yeast PST2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228166	SP_D4	Plasmid contains the secretion signal peptide sequence of the human LPAL2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228167	SP_D5	Plasmid contains the secretion signal peptide sequence of the human LUZP2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228168	SP_D6	Plasmid contains the secretion signal peptide sequence of the human DEFB131A gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228169	SP_E1	Plasmid contains the secretion signal peptide sequence of the yeast MEL5 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228170	SP_E2	Plasmid contains the secretion signal peptide sequence of the yeast FLO11 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228171	SP_E3	Plasmid contains the secretion signal peptide sequence of the yeast DAN4 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228172	SP_E4	Plasmid contains the secretion signal peptide sequence of the human FA12 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228173	SP_E5	Plasmid contains the secretion signal peptide sequence of the human CCL13 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228174	SP_E6	Plasmid contains the secretion signal peptide sequence of the human EPYC gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228175	SP_F1	Plasmid contains the secretion signal peptide sequence of the yeast CCW12 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228176	SP_F2	Plasmid contains the secretion signal peptide sequence of the yeast EXG1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228177	SP_F3	Plasmid contains the secretion signal peptide sequence of the yeast ECM33 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228178	SP_F4	Plasmid contains the secretion signal peptide sequence of the human CCL2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228179	SP_F5	Plasmid contains the secretion signal peptide sequence of the human MMP11 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228180	SP_F6	Plasmid contains the secretion signal peptide sequence of the human PDGFC gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228181	SP_G1	Plasmid contains the secretion signal peptide sequence of the yeast DSE2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228182	SP_G2	Plasmid contains the secretion signal peptide sequence of the yeast PRY1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228183	SP_G3	Plasmid contains the secretion signal peptide sequence of the yeast AWA1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228184	YEE_GFP	Yeast episomal expression vector with type '234' GFP drop out site. For use in assembling protein expression cassettes via golden gate assembly.
228185	YEE_RFP	Yeast episomal expression vector with type '234' RFP drop out site. For use in assembling protein expression cassettes via golden gate assembly.
228186	SP_G6	Plasmid contains the secretion signal peptide sequence of the human EPGN gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228187	SP_H1	Plasmid contains the secretion signal peptide sequence of the yeast SPR1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228188	SP_H2	Plasmid contains the secretion signal peptide sequence of the yeast HPF1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228189	SP_H3	Plasmid contains the secretion signal peptide sequence of the yeast CIS3 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228190	SP_H4	Plasmid contains the secretion signal peptide sequence of the human CCL8 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228191	SP_H5	Plasmid contains the secretion signal peptide sequence of the human SDC4 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
228192	SP_H6	Plasmid contains the secretion signal peptide sequence of the human BPIB4 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
238899	HSA_3b*_AP	Plasmid insert contains a gene sequence for Human serum albumin for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
238900	C-term_aMFx1 (4a)	Plasmid insert contains the aMFx1 C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
238901	C-term_aMFx1_noKREAEA (4a)	Plasmid insert contains the aMFx1 noKREAEA C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
238902	C-term_aMFx4 (4a)	Plasmid insert contains the aMFx4 C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
238903	C-term_TEV_aMFx1 (4a)	Plasmid insert contains the aMFx1 C-terminal tag sequence with the TEV cleavage site for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
238904	twinStrep_FLAG_aMFx1 (4a)	Plasmid insert contains the aMFx1, FLAG and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
238905	twinStrep_HA_aMFx1 (4a)	Plasmid insert contains the aMFx1, HA and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
238906	twinStrep_aMFx1 (4a)	Plasmid insert contains the aMFx1 and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
238907	C-term_C-myc (4a)	Plasmid insert contains the C-myc C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).