High-throughput optimisation of protein secretion in yeast via an engineered biosensor.
Cleaver A, Luo R, Smith OB, Murphy L, Schwessinger B, Brock J
Trends Biotechnol. 2025 Apr;43(4):838-867. doi: 10.1016/j.tibtech.2024.11.010. Epub 2024 Dec 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228145 | SP_A1 | Plasmid contains the secretion signal peptide sequence of the yeast MEL1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228146 | SP_A2 | Plasmid contains the secretion signal peptide sequence of the yeast (S. uvarum) SPR1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228147 | SP_A3 | Plasmid contains the secretion signal peptide sequence of the human ECM33 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228148 | SP_A4 | Plasmid contains the secretion signal peptide sequence of the human CHADL gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228149 | SP_A5 | Plasmid contains the secretion signal peptide sequence of the human GDF8 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228150 | SP_A6 | Plasmid contains the secretion signal peptide sequence of the human COLL1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228151 | SP_B1 | Plasmid contains the secretion signal peptide sequence of the yeast INV1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228152 | SP_B2 | Plasmid contains the secretion signal peptide sequence of the yeast FIG2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228153 | SP_B3 | Plasmid contains the secretion signal peptide sequence of the yeast (strain YJM789) PIR1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228154 | SP_B4 | Plasmid contains the secretion signal peptide sequence of the human LRP8 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228155 | SP_B5 | Plasmid contains the secretion signal peptide sequence of the human CCL7 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228156 | SP_B6 | Plasmid contains the secretion signal peptide sequence of the human CREG1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228157 | SP_C1 | Plasmid contains the secretion signal peptide sequence of the yeast MEL6 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228158 | SP_C2 | Plasmid contains the secretion signal peptide sequence of the yeast (S. mikatae) MEL gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228159 | SP_C3 | Plasmid contains the secretion signal peptide sequence of the yeast (strain ATCC 204508 / S288c) PIR1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228160 | SP_C4 | Plasmid contains the secretion signal peptide sequence of the human AFAM gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228161 | SP_C5 | Plasmid contains the secretion signal peptide sequence of the human STRCL gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228162 | SP_C6 | Plasmid contains the secretion signal peptide sequence of the human NELL1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228163 | SP_D1 | Plasmid contains the secretion signal peptide sequence of the yeast MEL2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228164 | SP_D2 | Plasmid contains the secretion signal peptide sequence of the yeast DAN1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228165 | SP_D3 | Plasmid contains the secretion signal peptide sequence of the yeast PST2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228166 | SP_D4 | Plasmid contains the secretion signal peptide sequence of the human LPAL2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228167 | SP_D5 | Plasmid contains the secretion signal peptide sequence of the human LUZP2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228168 | SP_D6 | Plasmid contains the secretion signal peptide sequence of the human DEFB131A gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228169 | SP_E1 | Plasmid contains the secretion signal peptide sequence of the yeast MEL5 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228170 | SP_E2 | Plasmid contains the secretion signal peptide sequence of the yeast FLO11 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228171 | SP_E3 | Plasmid contains the secretion signal peptide sequence of the yeast DAN4 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228172 | SP_E4 | Plasmid contains the secretion signal peptide sequence of the human FA12 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228173 | SP_E5 | Plasmid contains the secretion signal peptide sequence of the human CCL13 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228174 | SP_E6 | Plasmid contains the secretion signal peptide sequence of the human EPYC gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228175 | SP_F1 | Plasmid contains the secretion signal peptide sequence of the yeast CCW12 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228176 | SP_F2 | Plasmid contains the secretion signal peptide sequence of the yeast EXG1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228177 | SP_F3 | Plasmid contains the secretion signal peptide sequence of the yeast ECM33 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228178 | SP_F4 | Plasmid contains the secretion signal peptide sequence of the human CCL2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228179 | SP_F5 | Plasmid contains the secretion signal peptide sequence of the human MMP11 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228180 | SP_F6 | Plasmid contains the secretion signal peptide sequence of the human PDGFC gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228181 | SP_G1 | Plasmid contains the secretion signal peptide sequence of the yeast DSE2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228182 | SP_G2 | Plasmid contains the secretion signal peptide sequence of the yeast PRY1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228183 | SP_G3 | Plasmid contains the secretion signal peptide sequence of the yeast AWA1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228184 | YEE_GFP | Yeast episomal expression vector with type '234' GFP drop out site. For use in assembling protein expression cassettes via golden gate assembly. |
| 228185 | YEE_RFP | Yeast episomal expression vector with type '234' RFP drop out site. For use in assembling protein expression cassettes via golden gate assembly. |
| 228186 | SP_G6 | Plasmid contains the secretion signal peptide sequence of the human EPGN gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228187 | SP_H1 | Plasmid contains the secretion signal peptide sequence of the yeast SPR1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228188 | SP_H2 | Plasmid contains the secretion signal peptide sequence of the yeast HPF1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228189 | SP_H3 | Plasmid contains the secretion signal peptide sequence of the yeast CIS3 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228190 | SP_H4 | Plasmid contains the secretion signal peptide sequence of the human CCL8 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228191 | SP_H5 | Plasmid contains the secretion signal peptide sequence of the human SDC4 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |
| 228192 | SP_H6 | Plasmid contains the secretion signal peptide sequence of the human BPIB4 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites). |