Template plasmids optimized for deletion of multiple genes in yeast Saccharomyces cerevisiae.
Feng Y-H, Song J-Z, Zhu J, Xie Z
Microbiol Spectr. 2024 Oct 10:e0132024. doi: 10.1128/spectrum.01320-24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228256 | SJZ2-TRP1 | Template plasmid for yeast gene knockout, uses auxotrophic marker TRP1 (Kluyveromyces lactis) |
| 228257 | SJZ4-NAT | Template plasmid for yeast gene knockout, confers antibiotic resistance to nourseothricin |
| 228258 | SJZ5-HPH | Template plasmid for yeast gene knockout, confers antibiotic resistance to hygromycin B |
| 228259 | SJZ6-HIS5 | Template plasmid for yeast gene knockout, uses auxotrophic marker his5 (Schizosaccharomyces Pombe) (complements S.c. his3) |
| 228260 | SJZ7-KAN | Template plasmid for yeast gene knockout, confers antibiotic resistance to G418 |
| 228261 | SJZ9-amdS | Template plasmid for yeast gene knockout, allows the use of acetamide as sole nitrogen source |
| 228262 | SJZ10-LEU2 | Template plasmid for yeast gene knockout, uses auxotrophic marker LEU2 (Kluyveromyces lactis) |
| 228263 | SJZ1 | Plasmid backbone |
| 228264 | SJZ2 | Plasmid backbone |
| 228265 | SJZ4 | Plasmid backbone |
| 228267 | SJZ6 | Plasmid backbone |
| 228268 | SJZ7 | Plasmid backbone |
| 228269 | SJZ9 | Plasmid backbone |
| 228270 | SJZ10 | Plasmid backbone |