Designing strong inducible synthetic promoters in yeasts.

Tominaga M, Shima Y, Nozaki K, Ito Y, Someda M, Shoya Y, Hashii N, Obata C, Matsumoto-Kitano M, Suematsu K, Matsukawa T, Hosoya K, Hashiba N, Kondo A, Ishii J
Nat Commun. 2024 Dec 19;15(1):10653. doi: 10.1038/s41467-024-54865-z. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
228284	pKN108	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228285	pYI277	Constitutive EGFP expression
228286	pMT478	Constitutive mutant rPhlTA expression with strong promoter
228287	pMT856	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228288	pMT899	Constitutive mutant rPhlTA expression with strong promoter
228289	pMT949	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228290	pMT950	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228291	pMT951	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228292	pMT952	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228293	pMT957	Constitutive mutant PhlTA expression with strong promoter
228294	pMT958	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228295	pMT959	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228296	pMT960	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228297	pMT961	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228298	pMT1014	Homoserine lactone (HSL)-regulatable EGFP expression
228299	pMT1026	Constitutive expression of PhlTA mutant with weak promoter and terminator
228300	pMT1035	Constitutive expression of LuxTA mutant with strong promoter
228301	pMT1219	2,4-Diacetylphloroglucinol (DAPG)-regulatable expression of SARS-CoV-2 RBD omicron variant
228302	pMT1328	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228303	pMT1341	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228304	pMT1342	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228305	pMT1343	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228306	pMT1344	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228307	pMT1345	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228308	pMT1346	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228309	pMT1347	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228310	pMT1348	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228311	pMT1349	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228312	pMT1350	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228313	pMT1351	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228314	pMT1375	Constitutive expression of mutant rTetTA
228315	pMT1419	Doxycycline-regulatable EGFP expression
228316	pMT1420	Homoserine lactone (HSL)-regulatable EGFP expression
228317	pMT1434	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228318	pMT1449	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228319	pMT1461	Doxycycline-regulatable ALX-0081 expression
228320	pMT1473	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228321	pMT1474	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228322	pMT1475	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228323	pMT1481	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228324	pMT1482	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228325	pMT1483	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228326	pMT1487	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228327	pMT1821	Constitutive mutant rTetTA expression
228328	pMT1856	Homoserine lactone (HSL)-regulatable EGFP expression
228329	pMT1857	Homoserine lactone (HSL)-regulatable EGFP expression
228330	pMT1858	Homoserine lactone (HSL)-regulatable EGFP expression
228331	pMT1876	Homoserine lactone (HSL)-regulatable EGFP expression
228332	pMT1877	Homoserine lactone (HSL)-regulatable EGFP expression
228333	pMT1883	Homoserine lactone (HSL)-regulatable EGFP expression
228334	pMT1980	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228335	pMT1981	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228336	pMT2013	Doxycycline-regulatable EGFP expression
228337	pMT2076	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228338	pMT2077	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228339	pMT2080	Doxycycline-regulatable EGFP expression
228340	pMT2081	Doxycycline-regulatable EGFP expression
228341	pMT2086	Doxycycline-regulatable EGFP expression
228342	pMT2092	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228343	pMT2093	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228344	pMT2094	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228345	pMT2095	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228346	pMT2096	2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
228347	pYI2165	2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0171 expression
228348	pYI2206	Constitutive rPhlTA mutant expression
228349	pYI2216	2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0081 expression
228350	pYI2292	Methanol-inducible ALX-0171 expression
228351	pYI2293	Constitutive ALX-0081 expression
228352	pYI2294	Methanol-inducible ALX-0081 expression