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Engineered Stop and Go T7 RNA Polymerases.

Baumer ZT, Newton MS, Lofstrand L, Carpio Paucar GN, Farny NG, Whitehead TA
ACS Synth Biol. 2024 Dec 20;13(12):4165-4174. doi: 10.1021/acssynbio.4c00627. Epub 2024 Nov 28. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
228506pZB260_pT7-STR-H3U3Bacterial Hybrid Reporter Plasmid, pT7-HIS3/URA3
228507pZB578_pB1H1_T7-RNAP_LARP-IBacterial Hybrid Driver Plasmid
228508pZB573_pB1H1_T7-RNAP_LARP-I5CHO_1Bacterial Hybrid Driver Plasmid
228510pZB604_pT7-sfGFPBacterial Hybrid Reporter Plasmid (sfGFP), pT7-sfGFP
228511pZB641_pT7_Lysin-KU1T7 RNAP inducible Lysin KU1 Expression
228512US0 pyrF-/hisB-/tnaA-Indole-responsive Growth Selection
228513MG1655 tnaA-Indole-responsive Phage Infection Assay using LARP and T7 Phage with gp1 knock-out

Antibodies from Article